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In the present work, ZnSe nanoparticles were synthesized at pH=11.2 by aqueous method at different microwave irradiation times, and their antimicrobial properties against the Staphylococcus aureus and Bacillus cereus were investigated. X-ray diffraction and SEM have been used as structural characterization techniques, and optical properties were studied by Uv-Vis spectroscopy;   Results of X-ray diffraction characterization and UV- visible spectroscopy, confirm the formation of nanoscale phase  within the range of 1.942-2.142 nm. By considering the small size of nanoparticles, Antibacterial property of these nanoparticles has been done by disc-diffusion method at the presence of Muller-Hinton–Agar solid culture. ZnSe nanoparticles owing negative charge interact with Thiol groups of protein cell membrane and perturb the cell operation, and it seems that surface attaching and penetration of nanoparticles on the bacteria cell to be the most important mechanism of antibacterial activity.  Generally, it can be stated that the penetration of nanoparticles to the cell walls of bacteria is the principal antibacterial feature of these nanoparticles. Among the studied bacteria, results showed that these nanoparticles have high antimicrobial property against the Bacillus cereus. Inhibition zone diameter against Staphylococcus aureus and Bacillus cereus for different samples, were respectively within the ranges of 8.96-16.86 mm and 9.37-14.34 mm, and certified the inverse relation between inhibition diameter and particle size.

Keywords: ZnSe nanocrystals (NCs), different microwave irradiation times, disc diffusion, antibacterial properties, gram positive bacteria.

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